Protein synthesis of the pro-inflammatory S100A8/A9 complex in plasmacytoid dendritic cells and cell surface S100A8/A9 on leukocyte subpopulations in systemic lupus erythematosus
2011

S100A8/A9 Complex in Systemic Lupus Erythematosus

Sample size: 63 publication 10 minutes Evidence: moderate

Author Information

Author(s): Christian Lood, Martin Stenström, Helena Tydén, Birgitta Gullstrand, Eva Källberg, Tomas Leanderson, Lennart Truedsson, Gunnar Sturfelt, Fredrik Ivars, Anders Bengtsson

Primary Institution: Lund University

Hypothesis

Can aberrant cell surface S100A8/A9 be observed in systemic lupus erythematosus (SLE) and can plasmacytoid dendritic cells synthesize S100A8/A9?

Conclusion

Patients with SLE had increased cell surface S100A8/A9, which may play a role in inflammation persistence.

Supporting Evidence

  • SLE patients had increased serum levels of S100A8/A9.
  • Cell surface S100A8/A9 was higher in patients with active disease compared to those with inactive disease.
  • Plasmacytoid dendritic cells were able to synthesize S100A8/A9.

Takeaway

This study found that a protein complex called S100A8/A9 is more present on certain immune cells in people with lupus, which might help explain why they have ongoing inflammation.

Methodology

Flow cytometry, confocal microscopy, and real-time PCR were used to measure S100A8/A9 levels in leukocyte subpopulations.

Potential Biases

Potential bias due to the recruitment of patients from a single center.

Limitations

The study did not explore the exact biological functions of S100A8/A9 in SLE.

Participant Demographics

{"age":{"median":42,"range":"19 to 81"},"gender":{"female_percentage":94,"male_percentage":6}}

Statistical Information

P-Value

p<0.0001

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/ar3314

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