Detecting Aflatoxin M1 in Dairy Products Using Nanobodies
Author Information
Author(s): Yi Li, Liu Haiyuan, Liu Yingda, Aiyisi, He Jing, Ming Liang
Primary Institution: Inner Mongolia Agricultural University
Hypothesis
Can a nanobody-based indirect competitive ELISA (icELISA) effectively detect aflatoxin M1 in dairy products?
Conclusion
The study developed a sensitive and rapid icELISA for detecting aflatoxin M1 in dairy products, achieving a limit of detection of 0.051 ng/mL.
Supporting Evidence
- The developed icELISA showed a limit of detection of 0.051 ng/mL.
- Validation studies in spiked samples resulted in recoveries of 95.40–111.33%.
- Nb M4 demonstrated high thermal stability compared to traditional monoclonal antibodies.
- The method exhibited negligible cross-reactivity with other mycotoxins.
Takeaway
Scientists created a new test to find a harmful substance in milk that can make people sick, and it works really well.
Methodology
An indirect competitive ELISA was developed using a nanobody for the detection of aflatoxin M1 in dairy products, validated with spiked samples.
Limitations
The sensitivity of the developed method is lower than that of some commercial kits.
Digital Object Identifier (DOI)
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