Environmental neurotoxin dieldrin induces apoptosis via caspase-3-dependent proteolytic activation of protein kinase C delta (PKCdelta): Implications for neurodegeneration in Parkinson's disease
2008
Dieldrin Causes Cell Death in Dopaminergic Neurons
publication
Evidence: high
Author Information
Author(s): Kanthasamy Anumantha G, Kitazawa Masashi, Yang Yongjie, Anantharam Vellareddy, Kanthasamy Arthi
Primary Institution: Iowa State University
Hypothesis
Dieldrin induces apoptosis in dopaminergic neuronal cells through a caspase-3-dependent mechanism.
Conclusion
Dieldrin triggers apoptosis in dopaminergic neurons by activating caspase-3 and cleaving protein kinase C delta.
Supporting Evidence
- Dieldrin exposure led to a significant increase in reactive oxygen species in N27 cells.
- Caspase-3 activity increased significantly after dieldrin treatment.
- Dieldrin caused the cleavage of protein kinase C delta, which is crucial for apoptosis.
- Flow cytometry showed a marked increase in apoptotic cells after dieldrin exposure.
Takeaway
Dieldrin, a pesticide, can make brain cells that produce dopamine die by causing them to self-destruct.
Methodology
The study used biochemical, immunochemical, and flow cytometric analyses on N27 dopaminergic neuronal cells to assess the effects of dieldrin.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website