Streptozocin Diabetes Increases TGF- in Rat Kidneys
Author Information
Author(s): Pascale H. Lane, Dustin M. Snelling, William J. Langer
Primary Institution: University of Nebraska Medical Center
Hypothesis
All isoforms of TGF- are increased in the diabetic rat kidney.
Conclusion
All three isoforms of TGF- are elevated in the kidneys of rats with streptozocin-induced diabetes.
Supporting Evidence
- Mean glomerular area was 25% greater in diabetic animals.
- TGF-I showed a 2-fold increase in message with a 3-fold increase in protein.
- TGF-2 mRNA increased approximately 6% while its protein doubled.
- TGF-3 message increased by 25%, producing a 35% increase in its protein.
Takeaway
When rats got diabetes from a medicine called streptozocin, their kidneys made more of a substance called TGF-, which might be important for kidney problems.
Methodology
Twenty male Sprague-Dawley rats were divided into two groups, one receiving streptozocin and the other saline, and various kidney and plasma measurements were taken after two weeks.
Limitations
The study does not address the specific roles of each TGF- isoform in diabetic nephropathy.
Participant Demographics
Twenty male Sprague-Dawley rats, approximately 150 gm body weight and 6 to 8 weeks old.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Want to read the original?
Access the complete publication on the publisher's website