Murine Gamma-herpesvirus Immortalization of Fetal Liver-Derived B Cells Requires both the Viral Cyclin D Homolog and Latency-Associated Nuclear Antigen
2011

How a Mouse Virus Can Turn B Cells into Cancer Cells

publication 10 minutes Evidence: moderate

Author Information

Author(s): Liang Xiaozhen, Paden Clinton R., Morales Francine M., Powers Ryan P., Jacob Joshy, Speck Samuel H.

Primary Institution: Emory University School of Medicine

Hypothesis

Infection of fetal liver-derived B cells with murine gammaherpesvirus 68 (MHV68) leads to their immortalization and differentiation into B plasmablasts.

Conclusion

The study shows that MHV68 can immortalize fetal liver-derived B cells, leading to the development of lymphomas in immunocompromised mice.

Supporting Evidence

  • MHV68 infection of fetal liver cells leads to their immortalization and differentiation into B plasmablasts.
  • Immortalized B cell lines can establish lymphomas in immunocompromised mice.
  • Viral cyclin D homolog and latency-associated nuclear antigen are required for B cell immortalization.

Takeaway

Researchers found that a virus can change baby mouse blood cells into cancer cells that can grow forever in a lab and cause tumors in sick mice.

Methodology

Fetal liver cells from day 15 embryos were infected with MHV68 and cultured to generate immortalized B cell lines.

Limitations

The study primarily focuses on a specific mouse model, which may not fully represent human gammaherpesvirus infections.

Participant Demographics

Fetal liver-derived B cells from day 15 mouse embryos were used.

Digital Object Identifier (DOI)

10.1371/journal.ppat.1002220

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