Expression and Function of Osteopontin in Vascular Adventitial Fibroblasts and Pathological Vascular Remodeling
2011

Osteopontin's Role in Vascular Adventitial Fibroblasts and Vascular Remodeling

Sample size: 6 publication Evidence: moderate

Author Information

Author(s): Jin Xin, Fu Guo-xiang, Li Xiao-dong, Zhu Ding-liang, Gao Ping-jin

Primary Institution: State Key Laboratory of Medical Genomics and Shanghai Key Laboratory of Vascular Biology, Shanghai JiaoTong University School of Medicine, Ruijin Hospital, Shanghai, China

Hypothesis

OPN is upregulated in vascular advential by renin-angiotensin-aldosterone system, which thus plays an important role in neointima formation.

Conclusion

The study concluded that upregulation of Osteopontin expression in adventitial fibroblasts might be important in the pathogenesis of vascular remodeling after arterial injury.

Supporting Evidence

  • Angiotensin II and aldosterone increased Osteopontin expression in adventitial fibroblasts in a time- and concentration-dependent manner.
  • MAPKs and AP-1 pathways were involved in Osteopontin upregulation.
  • Adventitial fibroblast migration stimulated by Angiotensin II and aldosterone required OPN expression.
  • Perivascular delivery of antisense oligonucleotide for Osteopontin suppressed neointimal formation post-injury.

Takeaway

Osteopontin helps cells in blood vessels move and grow when they are injured, which can lead to problems in the blood vessels.

Methodology

The study measured Osteopontin activation and its biological functions in cultured adventitial fibroblasts and injured rat carotid arteries induced by balloon angioplasty.

Participant Demographics

Male Sprague-Dawley rats aged 12-14 weeks.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0023558

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