Isolation and Genome Analysis of Foot-and-Mouth Disease Virus from Pigs
Author Information
Author(s): Yang Xin, Zhou Ying-shun, Wang Hong-ning, Zhang Yi, Wei Kun, Wang Ting
Primary Institution: School of Life Science, Sichuan University
Hypothesis
Can a strain of foot-and-mouth disease virus serotype Asia1 be isolated from pigs in China?
Conclusion
This study reports the first isolation and identification of a strain of FMDV type Asia1 from naturally infected pigs.
Supporting Evidence
- The complete genome of the isolated strain was 8161 nucleotides long.
- The strain was designated as Asia1/WHN/CHA/06.
- Phylogenetic analysis showed that the strain is closely related to JS/CHA/05.
Takeaway
Researchers found a virus that usually affects cows and sheep in pigs for the first time, and they studied its genetic makeup.
Methodology
The study involved collecting samples from pigs, isolating the virus in cell cultures, and sequencing its complete genome.
Limitations
The study did not manage to sequence the poly(C) tract of the virus, which is often difficult to amplify.
Participant Demographics
Samples were collected from pigs in a farm in southwest China.
Digital Object Identifier (DOI)
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