A simple flow-injection method for the determination of blood glucose using a Technicon immobilized enzyme coil
1989
Simple Method for Measuring Blood Sugar
Sample size: 6
publication
Evidence: moderate
Author Information
Author(s): Sveinbj6rn Gizurarson
Primary Institution: Royal Danish School of Pharmacy
Hypothesis
The study investigates the effectiveness of a flow-injection system with an immobilized enzyme coil for glucose determination.
Conclusion
The flow-injection system is simple and practical for periodic glucose analysis.
Supporting Evidence
- The detection limit for glucose was found to be 0.10 mM.
- The method achieved a correlation coefficient of 0.994.
- The mean coefficient of variation for glucose measurements was 1.5%.
- The system can perform up to 40 determinations per hour with dilution.
Takeaway
This study shows a new way to measure blood sugar that is easy to use and can test many samples quickly.
Methodology
The method involved using a flow-injection system with an immobilized enzyme coil and UV detection to measure glucose levels in serum and pure glucose solutions.
Limitations
The method's capacity is limited by the viscosity of the samples, requiring dilution for higher throughput.
Want to read the original?
Access the complete publication on the publisher's website