Role of the AP2 β-Appendage Hub in Recruiting Partners for Clathrin-Coated Vesicle Assembly
Author Information
Author(s): Eva M. Schmid, Marijn G. J. Ford, Anne Burtey, Gerrit J. K. Praefcke, Sew-Yeu Peak-Chew, Ian G. Mills, Alexandre Benmerah, Harvey T. McMahon
Primary Institution: Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom
Hypothesis
How does the AP2 β-appendage hub recruit partners for clathrin-coated vesicle assembly?
Conclusion
The study identifies that the AP2 β-appendage acts as a hub for recruiting accessory proteins necessary for clathrin-coated vesicle formation.
Supporting Evidence
- Mass spectrometry identified over 20 different proteins interacting with the β2-appendage.
- The β-appendage collaborates with the α-appendage to bind a similar subset of ligands.
- Clathrin polymerization displaces accessory proteins during coated pit maturation.
- Structural analysis elucidated modes of ligand interactions with the β-appendage.
- Mutagenesis studies confirmed critical residues for ligand binding on the β-appendage.
Takeaway
The AP2 β-appendage helps gather proteins needed to form tiny bubbles in cells that transport materials, like a team working together to build a toy.
Methodology
Mass spectrometry and crystallization were used to identify proteins recruited by the AP2 β-appendage.
Limitations
The study may not account for all possible interactions in different cellular contexts.
Digital Object Identifier (DOI)
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