Timing of Breast Cancer Biopsies Affects Gene Expression
Author Information
Author(s): Wong Vietty, Wang Dong-Yu, Warren Keisha, Kulkarni Supriya, Boerner Scott, Done Susan Jane, Leong Wey Liang
Primary Institution: University Health Network, Toronto, Canada
Hypothesis
Does the timing of tissue acquisition (pre- or post-surgery) influence DNA microarray results in breast cancer?
Conclusion
The timing of fine needle aspiration biopsies can significantly affect gene expression profiles in breast cancer, particularly for FOS-related genes linked to hypoxia.
Supporting Evidence
- 12 genes were found to be differentially expressed between FNABs taken before and after surgery.
- FOS-related genes were linked to early hypoxia pathways.
- The study suggests that timing of tissue acquisition must be considered in future studies.
Takeaway
When doctors take samples of breast cancer tissue before or after surgery, it can change the way certain genes behave, especially those related to low oxygen levels.
Methodology
Paired fine needle aspiration biopsies were taken from 16 patients before and after surgery, and gene expression was analyzed using DNA microarrays.
Potential Biases
Potential bias from varying ischemic times and handling of samples.
Limitations
The study may not account for all variables affecting gene expression, such as anesthesia effects or stress from surgery.
Participant Demographics
16 patients with palpable invasive breast cancer scheduled for surgery.
Statistical Information
P-Value
0.002 and 0.006
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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