HPLC Method for Analyzing Cephalosporins in Pharmaceuticals and Water
Author Information
Author(s): Alqahtani Ali, Alqahtani Taha, Al Fatease Adel, Tolba Enas H.
Primary Institution: King Khalid University
Hypothesis
The study aims to develop a green HPLC method for the simultaneous determination of four cephalosporin antibiotics in pharmaceutical formulations and water samples.
Conclusion
The developed HPLC method effectively separates four cephalosporin antibiotics with good resolution in under 6 minutes and is environmentally friendly.
Supporting Evidence
- The method achieved baseline separation of the four cephalosporins.
- The AGREE score of the method was 0.75, indicating its environmental friendliness.
- The method was validated according to ICH guidelines for accuracy and precision.
- Recovery values for spiked tap water samples ranged from 98 to 102%.
Takeaway
Researchers created a new way to quickly check for certain medicines in water and drugs, which is better for the environment.
Methodology
The study used a Box–Behnken experimental design to optimize chromatographic parameters for HPLC analysis.
Limitations
Real water samples from different sources were not analyzed to evaluate the reliability of the method for environmental monitoring.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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