Src activates Abl to augment Robo1 expression in order to promote tumor cell migration
2010
Src Activates Abl to Increase Robo1 and Help Tumor Cells Move
publication
Evidence: moderate
Author Information
Author(s): Khusial P. Raaj, Vadla Bhaskar, Krishnan Harini, Ramlall Trudy F., Shen Yongquan, Ichikawa Hitoshi, Geng Jian-Guo, Goldberg Gary S.
Primary Institution: University of Medicine and Dentistry of New Jersey
Hypothesis
Src activates Abl to stabilize Robo1 expression in order to promote tumor cell migration.
Conclusion
Src activates Abl to stabilize Robo1, which promotes tumor cell migration.
Supporting Evidence
- Src transformed cells expressed over 4 times more Slit2 mRNA than nontransformed cells.
- Src decreased Robo1 mRNA levels while increasing Robo1 protein levels.
- Inhibition of Abl kinase activity reduced Robo1 expression and cell migration.
- Src transformed cells migrated significantly more than nontransformed cells.
- Inhibition of Robo1 signaling suppressed the migration of Src transformed cells.
Takeaway
This study shows that a protein called Src helps another protein, Abl, to keep Robo1 stable, which helps cancer cells move around.
Methodology
The study used expression microarrays, qRT-PCR, Western blotting, and immunoprecipitation to analyze protein levels and cell migration.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
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