Metabolomics in Early Alzheimer's Disease: Identification of Altered Plasma Sphingolipidome Using Shotgun Lipidomics
2011

Lipid Changes in Early Alzheimer's Disease

Sample size: 52 publication Evidence: moderate

Author Information

Author(s): Han Xianlin, Rozen Steve, Boyle Stephen H., Hellegers Caroline, Cheng Hua, Burke James R., Welsh-Bohmer Kathleen A., Doraiswamy P. Murali, Kaddurah-Daouk Rima

Primary Institution: Duke University Medical Center

Hypothesis

The plasma lipidome is altered in early Alzheimer's disease.

Conclusion

The study found significant reductions in sphingomyelin levels and increases in ceramide levels in the plasma of Alzheimer's disease patients compared to controls.

Supporting Evidence

  • Plasma levels of sphingolipids were significantly altered in Alzheimer's disease.
  • Eight sphingomyelin species were significantly lower in AD patients compared to controls.
  • Two ceramide species were significantly higher in AD patients.
  • MMSE scores correlated with altered mass levels of specific sphingolipids.
  • Ratios of ceramide to sphingomyelin species differed significantly between AD patients and controls.

Takeaway

This study looked at blood samples from people with Alzheimer's disease and found that certain fats in their blood were different from those without the disease, which might help in early diagnosis.

Methodology

The study used multi-dimensional mass spectrometry-based shotgun lipidomics to analyze plasma samples from Alzheimer's patients and cognitively normal controls.

Potential Biases

Possible biases from differences in age, diet, medical status, and concomitant medications among participants.

Limitations

The study had a small sample size and potential biases due to participant selection and other confounding factors.

Participant Demographics

26 Alzheimer's patients and 26 cognitively normal controls, with a mean age of 77.2 for AD patients and 73.0 for controls.

Statistical Information

P-Value

p<0.004

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0021643

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