Protective Effects of Indole-3-Acetic Acid and Chenodeoxycholic Acid Against Kidney Injury from Valproate
Author Information
Author(s): Ahlam M. Alhusaini, Wedad Sarawi, Noor Mukhtar, Danah Aljubeiri, Amjad S. Aljarboa, Hessa Alduhailan, Faris Almutairi, Raeesa Mohammad, Muhammad Atteya, Iman Hasan
Primary Institution: King Saud University
Hypothesis
Indole-3-acetic acid (IAA) and chenodeoxycholic acid (CDCA) can alleviate renal injury induced by valproate (VPA) by modulating the Nrf2/HO-1 and cytoglobin pathways.
Conclusion
IAA and CDCA alleviate kidney injury induced by VPA by reducing inflammation and enhancing antioxidant capacity in renal tissue.
Supporting Evidence
- VPA significantly increased serum creatinine and BUN levels, indicating kidney injury.
- Treatment with IAA or CDCA significantly reduced serum creatinine and BUN levels compared to VPA alone.
- IAA and CDCA improved renal histopathology by reducing cell degeneration.
- Both treatments downregulated inflammatory cytokines IL-6, IL-1β, and TNF-α in kidney tissues.
- IAA and CDCA restored antioxidant markers GSH and SOD levels in VPA-treated rats.
- Both compounds upregulated the Nrf2/HO-1 signaling pathway in renal tissues.
Takeaway
This study shows that two substances, IAA and CDCA, can help protect the kidneys from damage caused by a medicine called valproate.
Methodology
Rats were divided into four groups: control, VPA group, and two treatment groups receiving IAA or CDCA after VPA administration for 3 weeks, followed by biochemical and histopathological analyses.
Limitations
The study's sample size was limited to 24 rats, which may affect the generalizability of the findings.
Participant Demographics
Adult male Wistar rats, average weight 160 ± 20 g.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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