Caerulein-induced acute pancreatitis in mice with Reg/PAP gene overexpression
Author Information
Author(s): Oxana Norkina, Rolf Graf, Philippe Appenzeller, Robert C De Lisle
Primary Institution: University of Kansas School of Medicine
Hypothesis
The constitutively elevated expression of Reg/PAP genes in the CF mouse pancreas would lead to a less severe course of experimentally-induced acute pancreatitis.
Conclusion
The severity of caerulein-induced pancreatitis is not reduced in the CF mouse despite high levels of Reg/PAP gene expression.
Supporting Evidence
- Serum amylase levels were significantly increased in both wild type and CF mice after caerulein injection.
- Pancreatic edema was maximal at 7 hours in wild type mice.
- MPO activity increased significantly in both wild type and CF pancreas after caerulein administration.
Takeaway
Researchers wanted to see if mice with a certain gene would have less severe pancreatitis, but they found that it didn't help at all.
Methodology
The study used the caerulein model to induce acute pancreatitis in wild type and CF mice, measuring serum amylase, pancreatic water content, and myeloperoxidase activity.
Limitations
The study did not account for potential differences in pancreatic tissue content of digestive enzymes between CF and wild type mice.
Participant Demographics
Mice of both sexes, aged 6–7 weeks.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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