Profile of MicroRNAs following Rat Sciatic Nerve Injury by Deep Sequencing: Implication for Mechanisms of Nerve Regeneration
2011

MicroRNA Changes After Rat Sciatic Nerve Injury

Sample size: 30 publication 10 minutes Evidence: moderate

Author Information

Author(s): Yu Bin, Zhou Songlin, Wang Yongjun, Ding Guohui, Ding Fei, Gu Xiaosong

Primary Institution: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, China

Hypothesis

The study investigates the alterations in microRNA expression following sciatic nerve injury in rats and their implications for nerve regeneration.

Conclusion

The study found that specific microRNAs are significantly altered after nerve injury and may play important roles in nerve regeneration.

Supporting Evidence

  • 201 and 225 known microRNAs showed significant expression variance in dorsal root ganglia and sciatic nerve tissues after injury.
  • Bioinformatics analysis indicated that the potential targets for these microRNAs are involved in nerve regeneration.
  • Specific microRNAs were identified that may regulate key processes in nerve repair.

Takeaway

When rats hurt their sciatic nerve, certain tiny molecules called microRNAs change a lot, and these changes help the nerve heal.

Methodology

The study used deep sequencing to analyze microRNA expression in dorsal root ganglia and sciatic nerve tissues at various time points after injury.

Limitations

The study is limited to a rat model and may not fully translate to human nerve regeneration.

Participant Demographics

Adult male Sprague-Dawley rats, weighing 180-220 g.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0024612

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