Diffusion of Ethacrynic Acid and Sodium Fluorescein in Porcine Sclera
Author Information
Author(s): Lin Cheng-Wen, Wang Yong, Challa Pratap, Epstein David L., Yuan Fan
Primary Institution: Duke University
Hypothesis
Can ethacrynic acid be effectively delivered through the sclera without causing corneal toxicity?
Conclusion
Ethacrynic acid diffuses through the sclera more effectively than sodium fluorescein, indicating potential for transscleral drug delivery.
Supporting Evidence
- The diffusion coefficient of ethacrynic acid was found to be 48.5±15.1x10-7 cm2/s.
- Sodium fluorescein had a diffusion coefficient of 5.23±1.93x10-7 cm2/s.
- Binding of ethacrynic acid to scleral tissues was negligible.
- Diffusion coefficients were insensitive to cell damage and storage conditions.
Takeaway
This study shows that a drug called ethacrynic acid can move through the eye's outer layer better than another substance, which could help treat eye diseases without hurting the eye.
Methodology
An Ussing-type diffusion apparatus was used to measure the diffusion coefficients of ethacrynic acid and sodium fluorescein in fresh porcine sclera.
Limitations
The study was conducted using porcine sclera, which may not fully represent human scleral properties.
Participant Demographics
Fresh porcine sclera from enucleated eyes.
Statistical Information
Statistical Significance
p>0.05
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