How Dengue Virus Enters Cells
Author Information
Author(s): van der Schaar Hilde M., Rust Michael J., Chen Chen, van der Ende-Metselaar Heidi, Wilschut Jan, Zhuang Xiaowei, Smit Jolanda M.
Primary Institution: University Medical Center Groningen, University of Groningen
Hypothesis
The study investigates the cell entry pathway of Dengue virus using single-particle tracking.
Conclusion
Dengue virus enters cells exclusively via clathrin-mediated endocytosis and fuses primarily from within late endosomes.
Supporting Evidence
- 98% of DENV particles that fused with endosomes entered through clathrin-coated pits.
- DENV particles were tracked in real-time to observe their movement and entry process.
- Clathrin-mediated endocytosis was confirmed by the use of chlorpromazine, which inhibited viral entry.
- Membrane fusion events were primarily detected in Rab7-positive late endosomes.
Takeaway
Dengue virus uses a special door called clathrin-coated pits to get into cells, and it mostly opens this door inside a late endosome.
Methodology
The study used live-cell imaging and single-virus tracking to observe the entry and trafficking of Dengue virus particles in living cells.
Participant Demographics
The experiments were conducted using African green monkey kidney cells (BS-C-1) and HeLa cells.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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