Effects of Growth Factors on Tumor Growth and Hypoxia
Author Information
Author(s): J.T. Leith, G. Papa, L. Quaranto, S. Michelson
Primary Institution: Brown University School of Medicine
Hypothesis
Treatment of DLD-2 tumours with b FGF in vivo would decrease hypoxic fractions, while suramin treatment would increase hypoxic fractions.
Conclusion
Daily injections of b FGF decreased intratumour hypoxic fractions and increased volumetric growth rates, while suramin decreased growth rates and increased the extent of intratumour hypoxia.
Supporting Evidence
- b FGF treatment increased tumor growth by a factor of 1.5.
- Suramin treatment decreased tumor growth by a factor of 1.6.
- Control tumors had a hypoxic fraction of 42.9%.
- b FGF treatment reduced hypoxic fractions to 19.1%.
- Suramin treatment increased hypoxic fractions to 74.0%.
Takeaway
This study shows that giving certain growth factors can make tumors grow faster and change how much of the tumor is low on oxygen.
Methodology
Xenografted DLD-2 human colon tumors were treated with b FGF or suramin, and hypoxic fractions were measured using clonogenic excision assay.
Limitations
The hypoxic fractions were assayed in tumours of significantly different sizes.
Participant Demographics
Young adult male nude mice were used for the experiments.
Statistical Information
P-Value
p<0.05
Confidence Interval
95% confidence limits 34.2-52.1%
Statistical Significance
p<0.05
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