Antioxidant Activity of Caesalpinia crista Leaf Extract
Author Information
Author(s): Sourav Mandal, Bibhabasu Hazra, Rhitajit Sarkar, Santanu Biswas, Nripendranath Mandal
Primary Institution: Bose Institute
Hypothesis
The study aims to evaluate the antioxidant potential and reactive oxygen species (ROS) scavenging activity of the 70% methanol extract of Caesalpinia crista leaves.
Conclusion
The 70% methanol extract of C. crista leaves exhibits significant antioxidant and ROS scavenging activity, likely due to its high phenolic and flavonoid content.
Supporting Evidence
- The total antioxidant activity was evaluated with a trolox equivalent antioxidant capacity value of 0.546 ± 0.014.
- The extract showed significant scavenging activities for hydroxyl, superoxide, nitric oxide, singlet oxygen, and hypochlorous acid.
- The extract was found to be a potent iron chelator with an IC50 of 279.85 ± 4.72 μg/mL.
- The phenolic content was 50.23 ± 0.003 mg/mL gallic acid equivalent per 100 mg of plant extract.
- The flavonoid content was 106.83 ± 0.0003 mg/mL quercetin equivalent per 100 mg of plant extract.
- Treatment with the extract significantly increased the levels of antioxidant enzymes in mice.
Takeaway
This study shows that a plant called Caesalpinia crista can help protect our bodies from harmful things called free radicals, which can make us sick.
Methodology
The study involved in vitro tests to measure total antioxidant activity, ROS scavenging activities, iron chelating activity, and phenolic and flavonoid contents using a 70% methanolic extract of C. crista leaves.
Participant Demographics
Male Swiss albino mice were used for in vivo experiments.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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