Establishment of the 1st WHO International Standard for Plasmodium falciparum DNA
Author Information
Author(s): Padley David J, Heath Alan B, Sutherland Colin, Chiodini Peter L, Baylis Sally A
Primary Institution: National Institute for Biological Standards and Control
Hypothesis
Can a standardized preparation of Plasmodium falciparum DNA improve the consistency of NAT-based assays across laboratories?
Conclusion
The freeze-dried material AA was established as the 1st WHO International Standard for P. falciparum DNA NAT-based assays.
Supporting Evidence
- The study involved 14 laboratories testing four candidate standards.
- Sample AA was found to be highly stable and suitable for use as a standard.
- The mean log10 equivalents/ml for sample AA was determined to be 8.51.
Takeaway
Scientists created a special standard for testing malaria DNA to help labs get the same results when they check for the disease.
Methodology
Fourteen laboratories from 10 countries tested four candidate standards for P. falciparum DNA using qualitative and quantitative NAT-based assays.
Potential Biases
Potential bias due to the use of different assay methods and laboratory practices.
Limitations
Variability in assay sensitivity and differences in laboratory methods may affect results.
Participant Demographics
Laboratories from 10 different countries participated in the study.
Digital Object Identifier (DOI)
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