Dietary Fat and Cholesterol Metabolism in Mice Lacking P-glycoprotein
Author Information
Author(s): Sheila J. Thornton, Evelyn Wong, Stephen D. Lee, Kishor M. Wasan
Primary Institution: University of British Columbia
Hypothesis
How does dietary fat affect hepatic liver X receptor expression in P-glycoprotein deficient mice?
Conclusion
Mice lacking P-glycoprotein do not show changes in cholesterol storage or levels but have increased hepatic LXRα expression and higher fecal cholesterol concentration.
Supporting Evidence
- Mice lacking P-glycoprotein did not show differences in plasma total cholesterol or hepatic cholesterol storage.
- Increased hepatic LXRα protein expression was observed in P-glycoprotein deficient mice.
- Fecal cholesterol concentration was significantly higher in P-glycoprotein deficient mice compared to controls.
Takeaway
This study looked at how fat in food affects cholesterol in mice that can't use a certain protein. The mice without this protein didn't store more cholesterol but had more of a protein that helps manage cholesterol.
Methodology
The study used P-glycoprotein knockout mice and control mice fed either a normal fat or high fat diet for 12 weeks, measuring cholesterol levels and LXRα expression.
Limitations
The study did not measure the rate of bile formation, which could affect cholesterol efflux.
Participant Demographics
Male P-glycoprotein knockout mice and FVB control mice.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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