Phospholipase C Isozymes Are Deregulated in Colorectal Cancer – Insights Gained from Gene Set Enrichment Analysis of the Transcriptome
2011

Phospholipase C Isozymes and Colorectal Cancer

Sample size: 137 publication Evidence: high

Author Information

Author(s): Danielsen Stine A., Cekaite Lina, Ågesen Trude H., Sveen Anita, Nesbakken Arild, Thiis-Evensen Espen, Skotheim Rolf I., Lind Guro E., Lothe Ragnhild A.

Primary Institution: Oslo University Hospital

Hypothesis

The study aims to identify molecular networks and biological processes that are deregulated in colorectal cancer compared to normal colonic mucosa.

Conclusion

The study identified significantly deregulated pathways in colorectal cancer and validated the repression of PLCD1 and PLCE1 expression.

Supporting Evidence

  • The study analyzed two independent transcriptome datasets.
  • Eighty-two gene sets had significantly altered gene expression in both datasets.
  • The repression of PLCD1 and PLCE1 was validated in CRC biopsies and cell lines.

Takeaway

Researchers looked at genes in colorectal cancer and found some that are not working properly, which could help us understand the disease better.

Methodology

Gene Set Enrichment Analysis was applied to two independent transcriptome datasets.

Potential Biases

No specific bias risks were mentioned.

Limitations

The study's findings may be influenced by the risk of false significant associations due to multiple testing.

Participant Demographics

The study included 137 colorectal cancer samples and 10 normal colonic mucosa samples.

Statistical Information

P-Value

0.005 and 0.006

Statistical Significance

p≤0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0024419

Want to read the original?

Access the complete publication on the publisher's website

View Original Publication