X-box binding protein 1 and its role in herpesvirus reactivation
Author Information
Author(s): Lai Imogen Yi-Chun, Farrell Paul J., Kellam Paul
Primary Institution: University College London, MRC Centre for Molecular Virology
Hypothesis
Does X-box binding protein 1 (XBP-1) induce the expression of lytic cycle transactivators in co-infected primary effusion lymphoma?
Conclusion
XBP-1 specifically reactivates the KSHV lytic cycle in dually infected primary effusion lymphomas but does not induce EBV lytic cycle proteins.
Supporting Evidence
- XBP-1s induces K-RTA expression in PEL cells.
- XBP-1s does not induce BZLF1 or BRLF1 expression in PEL or BL cell lines.
- Chemical inducers do not lead to XBP-1 activation in PEL.
Takeaway
XBP-1 helps one virus wake up from sleep but doesn't help the other virus do the same in certain cancer cells.
Methodology
The study used lentiviral vectors to overexpress XBP-1s in various cell lines and assessed the expression of KSHV and EBV proteins through Western blotting and Q-RT-PCR.
Limitations
The study does not explore the effects of XBP-1s in all possible cell types and focuses primarily on specific lymphoma cell lines.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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