Loss of Xenopus tropicalis EMSY causes impairment of gastrulation and upregulation of p53
2011
The Role of EMSY in Xenopus Development
publication
Evidence: moderate
Author Information
Author(s): Rana Amer A., Roper Stephen J., Palmer Elizabeth A., Smith James C.
Primary Institution: Gurdon Institute, University of Cambridge
Hypothesis
What is the function of EMSY during amphibian development?
Conclusion
Loss of EMSY function impairs gastrulation and increases apoptosis in embryos.
Supporting Evidence
- Injection of morpholino oligonucleotides targeting XtEMSY disrupts gastrulation movements.
- Expression of regional markers is downregulated in embryos lacking EMSY.
- p53 expression is upregulated in embryos with reduced EMSY function.
Takeaway
EMSY is a protein that helps embryos develop properly, and when it's not working, the embryos have trouble moving and can die.
Methodology
Embryos were injected with antisense morpholino oligonucleotides to inhibit XtEMSY function and various gene expressions were analyzed.
Limitations
The study primarily focuses on the short form of XtEMSY and may not fully represent the protein's functions in all contexts.
Digital Object Identifier (DOI)
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