Creating Multi-Transgenic Pigs Using 2A Peptide
Author Information
Author(s): Deng Wei, Yang Dongshan, Zhao Bentian, Ouyang Zhen, Song Jun, Fan Nana, Liu Zhaoming, Zhao Yu, Wu Qinghong, Nashun Bayaer, Tang Jiangjing, Wu Zhenfang, Gu Weiwang, Lai Liangxue
Primary Institution: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Chinese Academy of Sciences, Guangzhou, China
Hypothesis
Can the viral 2A peptide be used to efficiently produce multi-transgenic pigs through a single round of nuclear transfer?
Conclusion
The study successfully demonstrated that multi-transgenic pigs can be generated using a 2A peptide-based double-promoter vector, with high levels of co-expression of four fluorescent proteins.
Supporting Evidence
- 54.3% of cell colonies co-expressed four fluorescent proteins at uniformly high levels.
- Eleven piglets were delivered, with seven co-expressing the four fluorescent proteins.
- The method allows for the efficient generation of multi-transgenic pigs in a single nuclear transfer.
Takeaway
Scientists found a way to make pigs that can show four different colors by using a special tool called the 2A peptide, which helps combine genes together.
Methodology
The study involved constructing a 2A peptide-based double-promoter expression vector, transfecting it into porcine fetal fibroblasts, and using somatic cell nuclear transfer to create transgenic pigs.
Limitations
The efficiency of gene expression varied, particularly for downstream genes in the polycistronic cassette.
Participant Demographics
The study involved transgenic piglets derived from seven recipient gilts.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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