Effects of Dietary Fat on Obesity in Mice
Author Information
Author(s): de Wit Nicole J. W., Boekschoten Mark V., Bachmair Eva-Maria, Hooiveld Guido J. E. J., de Groot Philip J., Rubio-Aliaga Isabel, Daniel Hannelore, Müller Michael
Primary Institution: Netherlands Nutrigenomics Centre, Top Institute Food and Nutrition, Wageningen, The Netherlands
Hypothesis
What are the dose-dependent effects of dietary fat on the development of obesity and intestinal gene expression in C57BL/6J mice?
Conclusion
The study concludes that a dietary fat intake above 20% triggers a shift towards an obese phenotype and alters gene expression in the small intestine.
Supporting Evidence
- Mice on a 30% and 45% fat diet gained significantly more weight than those on a control diet.
- Approximately 70% of differentially expressed genes showed a fat-dose dependent manner.
- The main shift in gene expression occurred between the 30% and 45% fat diets.
Takeaway
If mice eat too much fat, they gain weight and their intestines change how they work. Eating more than 20% fat in their diet makes them more likely to get fat.
Methodology
C57BL/6J mice were fed diets with varying fat content (10E%, 20E%, 30E%, 45E%) for four weeks, and gene expression was analyzed in the small intestine.
Limitations
The study primarily focuses on one mouse strain and may not generalize to other models of obesity.
Participant Demographics
C57BL/6J male mice, aged 12 weeks at the start of the study.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website