Generating Transgenic Mice Using Spermatogonial Stem Cells
Author Information
Author(s): Sehgal, Lalit, Thorat, Rahul, Khapare, Nileema, Mukhopadhaya, Amitabha, Sawant, Mugdha, Dalal, Sorab N.
Primary Institution: Advanced Centre for Treatment Research Education and Cancer, Tata Memorial Centre
Hypothesis
Can spermatogonial stem cells be effectively manipulated in vivo to generate transgenic mice?
Conclusion
The study successfully demonstrates a high success rate for generating transgenic mice through in vivo manipulation of spermatogonial stem cells.
Supporting Evidence
- All male pre-founder mice produced transgenic pups with an overall success rate of over 60%.
- The transgene was heritable and the pre-founder mice could be used in multiple mating experiments.
- This technology could be adapted to other laboratory animals.
- Transgenic pups were generated from mating experiments with three independently derived pre-founder mice.
Takeaway
Scientists found a new way to create mice with special genes by using cells that make sperm, and it worked really well!
Methodology
The study involved injecting lentiviruses into the testes of male mice to infect spermatogonial stem cells and then mating them to produce transgenic pups.
Potential Biases
Potential bias in the selection of successful transgenic lines and the efficiency of the viral transduction method.
Limitations
The study may not be applicable to all species and the long-term effects of the transgene were not fully explored.
Participant Demographics
Crl:CFW(SW) male mice were used in the experiments.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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