Lactobacillus johnsonii N6.2 Extracellular Vesicles Inhibit Murine Norovirus Replication
Author Information
Author(s): da Silva Danilo R., Sharjeel Asra B., Beliakoff Reagan, Teixeira Leandro D., Kima Peter E., Jones Melissa K., Gonzalez Claudio F., Lorca Graciela L.
Primary Institution: University of Florida
Hypothesis
Can Lactobacillus johnsonii N6.2-derived extracellular vesicles inhibit murine norovirus replication?
Conclusion
Lactobacillus johnsonii N6.2 extracellular vesicles significantly reduce murine norovirus replication in vitro and in vivo.
Supporting Evidence
- Lactobacillus johnsonii N6.2 EVs significantly decreased MNV-1 genome levels in macrophages.
- The SH3b2 domain of Sdp was identified as an effector molecule that activates the OAS pathway.
- Oral administration of L. johnsonii N6.2, EVs, and SH3b2 reduced MNV-1 titers in mice.
Takeaway
This study shows that tiny bubbles from a good bacteria called Lactobacillus can help stop a virus that makes mice sick.
Methodology
The study used RAW 264.7 macrophages and C57BL/6 mice to evaluate the antiviral effects of Lactobacillus johnsonii N6.2 extracellular vesicles and the Sdp-SH3b2 domain.
Potential Biases
Potential bias due to the authors' affiliations with the research institution.
Limitations
The study primarily focused on murine models, which may not fully represent human responses.
Participant Demographics
C57BL/6 mice were used in the in vivo experiments.
Statistical Information
P-Value
0.0042
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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