Cytokines in Graft vs. Host Disease in Mice
Author Information
Author(s): A. C. Knulst, G. J. M. Tibbe, C. BriI-Bazuin, E. G. Breedland, A. van Oudenaren, R. Benner, H. F. J. Savelkoul
Primary Institution: Erasmus University, Rotterdam, The Netherlands
Hypothesis
The study investigates the role of various cytokines in the development of acute lethal graft vs. host disease (GVHD).
Conclusion
The study found that IL-6, IFN-, and TNF- levels significantly increased in mice with acute GVHD, while IL-2 and IL-4 were undetectable.
Supporting Evidence
- Serum levels of IL-6, TNF-, and IFN- were significantly higher in allogeneically reconstituted mice compared to syngeneic controls.
- IL-2 and IL-4 levels were below the detection limit in all groups.
- Administration of anti-IL-2 and anti-IL-2 receptor antibodies decreased morbidity and mortality in GVHD.
Takeaway
This study looked at how certain proteins called cytokines affect a disease that can happen after a transplant. They found that some cytokines go up a lot when the disease is present.
Methodology
Mice were lethally irradiated and reconstituted with either allogeneic or syngeneic spleen cells, and serum and spleen cell cultures were analyzed for cytokine levels.
Participant Demographics
Mice were age matched and 12-18 weeks old.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
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