Metabolism of Aminoglutethimide in Humans
Author Information
Author(s): P.E. Goss, M. Jarman, L.J. Griggs
Primary Institution: Institute of Cancer Research
Hypothesis
The study aims to quantify hydroxylaminoglutethimide (HxAG) and confirm that its induction is AG-induced and increases with the duration of therapy.
Conclusion
The metabolism of aminoglutethimide to HxAG is an induced, major metabolic pathway that increases with time on therapy.
Supporting Evidence
- HxAG was not detectable on Day 1 but was abundant in the Day 14 extract.
- The ratio of HxAG to AG increased with time, indicating induced metabolism.
- All spot urine samples from patients on treatment were positive for HxAG.
Takeaway
This study shows that a drug called aminoglutethimide changes how the body processes it over time, which can affect how well it works.
Methodology
Quantification of HxAG, AG, and N-AcAG using high performance liquid chromatography (HPLC) and a colorimetric assay.
Limitations
The study faced challenges due to the instability of HxAG at room temperature, which affected the accuracy of measurements.
Participant Demographics
Participants included 50 post-menopausal women with metastatic breast cancer and 30 male and female out-patients.
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