DENV-1 Infection of Macrophages Induces Pyroptosis and Changes MicroRNA Expression
Author Information
Author(s): Zhang Qinyi, Yu Sicong, Yang Zhangnv, Wang Xingxing, Li Jianhua, Su Lingxuan, Zhang Huijun, Lou Xiuyu, Mao Haiyan, Sun Yi, Fang Lei, Yan Hao, Zhang Yanjun, Kawasaki Hideya, Russo Alessandro
Primary Institution: Zhejiang Provincial Center for Disease Control and Prevention
Hypothesis
DENV-1 infection induces pyroptosis in macrophages and alters microRNA expression profiles.
Conclusion
DENV-1 infection of macrophages leads to pyroptosis and significant changes in microRNA expression, which may contribute to inflammation.
Supporting Evidence
- DENV-1 infection significantly reduced macrophage survival rates.
- Infected macrophages showed increased LDH release, indicating cell membrane damage.
- IL-1β levels were significantly elevated in the supernatants of infected macrophages.
- Pyroptosis-related genes were activated in DENV-1 infected macrophages.
- 63 differentially expressed microRNAs were identified during DENV-1 infection.
Takeaway
When macrophages get infected by the dengue virus, they die in a special way that causes inflammation, and this also changes the levels of tiny molecules called microRNAs that help control inflammation.
Methodology
The study used high-throughput sequencing to analyze microRNA expression and various assays to measure cell viability, LDH release, and IL-1β levels in DENV-1 infected macrophages.
Limitations
The study focused only on DENV-1 and did not explore other serotypes; it was limited to in vitro experiments.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website