Genetic Diversity in Metarhizium anisopliae Isolates
Author Information
Author(s): Márquez Marcela, Iturriaga Enrique A, Quesada-Moraga Enrique, Santiago-Álvarez Cándido, Monte Enrique, Hermosa Rosa
Primary Institution: Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Universidad de Salamanca
Hypothesis
What is the genetic diversity present in an autochthonous collection of Metarhizium anisopliae isolates selected as biocontrol agents?
Conclusion
The study identified four insertion sites of group I introns in the LSU rDNA genes, establishing seven genotypes among the twenty-six biocontrol isolates of M. anisopliae.
Supporting Evidence
- The presence/absence of introns allowed the 26 isolates to be distributed into seven genotypes.
- Nine of the isolates tested showed no introns, while others had varying numbers of introns.
- The most frequent insertion sites were Ec1921 and Ec2449.
Takeaway
Scientists looked at a type of fungus that helps control pests and found different genetic types based on certain DNA markers.
Methodology
DNA fragments from LSU rDNA genes of 26 M. anisopliae isolates were amplified by PCR and analyzed for intron presence.
Limitations
The study was limited to a small collection of isolates and did not explore geographical correlations.
Digital Object Identifier (DOI)
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