Detecting Salmonella in Milk Using Lateral Flow Immunoassays
Author Information
Author(s): Byzova Nadezhda A., Safenkova Irina V., Gorbatov Alexey A., Biketov Sergey F., Dzantiev Boris B., Zherdev Anatoly V.
Primary Institution: A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences
Hypothesis
Can different formats of lateral flow immunoassays (LFIA) improve the detection of Salmonella Typhimurium in milk?
Conclusion
The study found that the common sandwich LFIA format was the most effective for detecting Salmonella Typhimurium in milk samples.
Supporting Evidence
- The detection limits for the LFIA formats were 3 × 10^4, 1 × 10^5, and 3 × 10^5 cells/mL.
- Format A showed recovery values of 70–110% for Salmonella detection in milk.
- Format A was the most sensitive and efficient technique for Salmonella detection in milk.
Takeaway
This study tested three ways to check milk for a germ called Salmonella. The easiest way worked best and helped find the germ quickly.
Methodology
The study compared three LFIA formats for detecting Salmonella Typhimurium in milk, measuring detection limits and assay times.
Limitations
Formats B and C were less effective in detecting Salmonella in milk compared to format A.
Digital Object Identifier (DOI)
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