Diversity of Ralstonia pickettii and Ralstonia insidiosa from Clinical and Environmental Sources
Author Information
Author(s): Ryan Michael P, Pembroke J Tony, Adley Catherine C
Primary Institution: University of Limerick
Hypothesis
What is the phenotypic and genotypic diversity of Ralstonia pickettii and Ralstonia insidiosa isolates from various sources?
Conclusion
Ralstonia pickettii and Ralstonia insidiosa isolates show limited diversity and can be distinguished primarily through species-specific PCR.
Supporting Evidence
- Fifty-nine isolates were analyzed from clinical, industrial, and environmental sources.
- Fifteen out of fifty-nine designated R. pickettii isolates were identified as R. insidiosa.
- Different banding patterns were observed for RAPD and BOX primers, but they did not vary significantly.
Takeaway
This study looked at bacteria from different places and found that they are very similar to each other, making it hard to tell them apart without special tests.
Methodology
The study used various phenotypic and genotypic methods including PCR, sequencing, and biochemical testing to analyze 59 isolates.
Limitations
The study may not capture the full diversity of Ralstonia species due to the limited number of isolates and environments sampled.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website