Antiviral Activity of Isoflavenes Against Vaccine-Derived Poliovirus
Author Information
Author(s): Shulman Lester M., Sofer Danit, Manor Yossi, Mendelson Ella, Balanant Jean, Salvati Anna Laura, Delpeyroux Francis, Fiore Lucia
Primary Institution: Central Virology Laboratory, Public Health Services Israel Ministry of Health
Hypothesis
Do highly evolved vaccine-derived polioviruses retain sensitivity to isoflavenes?
Conclusion
Isoflavenes effectively reduced viral loads in vitro against most highly-diverged vaccine-derived polioviruses.
Supporting Evidence
- Isoflavenes inhibited viral titers of Sabin-2 and nine of ten aVDPV2s by one to two log10.
- The tenth aVDPV2 isolate was unaffected by both isoflavenes.
- All aVDPV2 isolates reverted to a neurovirulent phenotype except one with specific amino acid substitutions.
Takeaway
Researchers tested two compounds to see if they could stop a type of poliovirus from growing, and they found that most of the viruses were still affected by the compounds.
Methodology
The study involved titering poliovirus isolates on HEp2C cells in the presence of isoflavenes and assessing neurovirulence in transgenic mice.
Limitations
The study did not evaluate the in vivo effectiveness of isoflavenes in humans.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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