The E. coli K-12 ORFeome: A Resource for Molecular Microbiology
Author Information
Author(s): Rajagopala Seesandra V, Yamamoto Natsuko, Zweifel Adrienne E, Nakamichi Tomoko, Huang Hsi-Kuang, Mendez-Rios Jorge David, Franca-Koh Jonathan, Boorgula Meher Preethi, Fujita Kazutoshi, Suzuki Ken-ichirou, Hu James C, Wanner Barry L, Mori Hirotada, Uetz Peter
Primary Institution: J Craig Venter Institute (JCVI)
Hypothesis
How can a comprehensive collection of Escherichia coli K-12 open reading frames (ORFs) facilitate functional genomics and protein interaction studies?
Conclusion
The E. coli ORFeome provides a useful resource for functional genomics and other areas of protein research in a highly flexible format.
Supporting Evidence
- The ORFeome collection includes 94% of the known E. coli K-12 ORFs.
- The library has been used for protein expression and protein interaction studies.
- Comparative analyses show conserved protein interactions across different bacterial species.
Takeaway
Scientists created a big library of E. coli genes that helps them study how proteins work together, making it easier to compare with other bacteria.
Methodology
The study involved constructing an ORFeome collection of 3974 E. coli K-12 ORFs using Gateway® entry vector pENTR/Zeo and validating the clones through DNA sequencing.
Limitations
The E. coli entry clone library lacks start and stop codons, which may limit certain types of expression studies.
Digital Object Identifier (DOI)
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