Development of a New Red Fluorescent Dye for Gene Analysis
Author Information
Author(s): Dar Mubasher, Giesler Theresa, Richardson Rob, Cai Christine, Cooper Mike, Lavasani Shahin, Kille Peter, Voet Thierry, Vermeesch Joris
Primary Institution: GE Healthcare
Hypothesis
Can a new dye, HyPer5, provide better stability than Cy5 under ozone and light exposure in microarray applications?
Conclusion
HyPer5 is a more stable alternative to Cy5, showing no signal loss under high ozone levels and light exposure.
Supporting Evidence
- HyPer5 showed no signal loss after exposure to 300 ppb ozone for up to 15 minutes.
- In photobleaching tests, HyPer5 demonstrated a 3-fold improvement in stability over Cy5.
- HyPer5 was able to detect chromosomal aberrations in patient samples effectively.
Takeaway
Scientists created a new dye called HyPer5 that doesn't break down in the presence of ozone or light, making it better for experiments than an older dye called Cy5.
Methodology
The study involved comparing the performance of HyPer5 and Cy5 under ozone exposure and light conditions using microarray experiments.
Limitations
The lower brightness of HyPer5 compared to Cy5 may require using more dye for effective results.
Digital Object Identifier (DOI)
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