Exit from Arsenite-Induced Mitotic Arrest Is p53 Dependent
2006
How p53 Affects Cell Division and Death in Response to Arsenic
Sample size: 4
publication
Evidence: moderate
Author Information
Author(s): Samuel C. McNeely, Xiaogiang Xu, B. Frazier Taylor, Wolfgang Zacharias, Michael J. McCabe Jr., J. Christopher States
Primary Institution: University of Louisville
Hypothesis
p53 plays a role in protecting cells from arsenite-induced mitotic arrest.
Conclusion
p53 promotes mitotic exit and leads to more extensive ID1 induction by arsenite.
Supporting Evidence
- Arsenite treatment delayed exit from G2 in p53(+) and p53(−) cells.
- p53(+) cells exited mitosis normally, while p53(−) cells had delayed exit.
- ID1 induction was more extensive in p53(+) cells after arsenite exposure.
- p53 deficiency led to increased sensitivity to arsenite-induced apoptosis.
Takeaway
This study shows that p53 helps cells escape a pause in division caused by arsenic, which can lead to cell death.
Methodology
The study used synchronized human fibroblast cells with regulated p53 expression to analyze the effects of arsenite on cell division.
Participant Demographics
Human fibroblast cell lines derived from a Li-Fraumeni patient.
Statistical Information
P-Value
<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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