Rep8: A Tissue-Specific Cofactor for p97 in Protein Degradation
Author Information
Author(s): Madsen Louise, Kriegenburg Franziska, Vala Andrea, Best Diana, Prag Søren, Hofmann Kay, Seeger Michael, Adams Ian R., Hartmann-Petersen Rasmus
Primary Institution: Department of Biology, University of Copenhagen, Copenhagen, Denmark
Hypothesis
Rep8 is a tissue-specific cofactor for the p97 ATPase involved in the degradation of misfolded proteins.
Conclusion
Rep8 tethers p97 to the ER membrane, facilitating the degradation of misfolded proteins.
Supporting Evidence
- Rep8 is highly tissue-specific and abundant in gonads.
- Knock-down of Rep8 expression leads to decreased p97 association with the ER membrane.
- Rep8 interacts directly with p97 via its UBX domain.
- Rep8 is a transmembrane protein localized to the ER membrane.
- Rep8 expression is primarily found in testes and ovaries.
Takeaway
Rep8 helps a protein called p97 do its job of getting rid of bad proteins in certain tissues, like the testis and ovary.
Methodology
The study involved yeast two-hybrid screening, co-precipitation experiments, and differential centrifugation to analyze the interaction and localization of Rep8 and p97.
Limitations
The study primarily focuses on the interaction in cell lines and may not fully represent in vivo conditions.
Participant Demographics
The study involved human cell lines and rat tissues.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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