Genome Assembly of the Barley Pathogen Pyrenophora teres f. teres
Author Information
Author(s): Ellwood Simon R, Liu Zhaohui, Syme Rob A, Lai Zhibing, Hane James K, Keiper Felicity, Moffat Caroline S, Oliver Richard P, Friesen Timothy L
Primary Institution: Curtin University
Hypothesis
Can paired-end Solexa sequencing effectively capture the genome of the barley fungal pathogen Pyrenophora teres f. teres?
Conclusion
The study successfully assembled the genome of Pyrenophora teres f. teres, revealing a wealth of predicted genes related to its pathogenicity.
Supporting Evidence
- The genome assembly was validated by comparison to BAC sequences and genetic mapping.
- The assembly contains 11,799 predicted gene models.
- Electrophoretic karyotyping confirmed the presence of at least nine chromosomes.
- The genetic map spans 2477.7 cM with 243 markers in 25 linkage groups.
- Non-ribosomal peptide synthetases and efflux pumps were found to be expanded in this pathogen.
Takeaway
Scientists sequenced the DNA of a fungus that causes disease in barley to better understand how it works and how to fight it.
Methodology
The genome was assembled using paired-end Solexa sequencing and validated through comparisons with BAC sequences and genetic mapping.
Limitations
The assembly may under-represent low-complexity regions and repetitive DNA due to the reliance on short paired-end reads.
Digital Object Identifier (DOI)
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