Identifying Imprinted Genes in Mice
Author Information
Author(s): Hagan John P., O'Neill Brittany L., Stewart Colin L., Kozlov Serguei V., Croce Carlo M.
Primary Institution: The Ohio State University Medical Center
Hypothesis
The study aims to identify and characterize novel imprinted genes that may contribute to mammalian phenotypes displaying parent of origin effects.
Conclusion
The study identified ten imprinted genes, including three paternally expressed protein coding genes and seven noncoding RNAs, expanding the understanding of genomic imprinting in mammals.
Supporting Evidence
- The study identified a maternally expressed transcript termed Imprinted RNA near Meg3/Gtl2 (Irm).
- The imprinting of three paternally expressed protein coding genes (Dlk1, Peg11, and Dio3) was confirmed.
- Seven noncoding RNAs were characterized by exclusive maternal expression.
Takeaway
The researchers found ten important genes in mice that are expressed differently depending on whether they come from the mother or father, which can affect how the mice grow and develop.
Methodology
Transcription profiling was performed on uniparentally derived cell lines, and gene expression was analyzed using Northern blotting and in situ hybridization.
Limitations
The study may not account for all imprinted genes in the region, and the findings are based on mouse models which may not fully translate to humans.
Digital Object Identifier (DOI)
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