The tep1 gene in Sinorhizobium meliloti and its role in nodulation of alfalfa
Author Information
Author(s): van Dillewijn Pieter, Sanjuán Juan, Olivares José, Soto María José
Primary Institution: Estación Experimental del Zaidín, CSIC, Granada, Spain
Hypothesis
What is the role of the tep1 gene in swarming and symbiosis in Sinorhizobium meliloti?
Conclusion
The tep1 gene encodes a protein that helps Sinorhizobium meliloti resist chloramphenicol and influences nodulation efficiency on alfalfa plants.
Supporting Evidence
- The tep1 mutant showed increased nodulation efficiency on alfalfa plants during the first days of interaction.
- N-acetyl glucosamine reduced nod gene expression and nodulation efficiency when present at high concentrations.
- The tep1 gene is linked to the efflux of compounds that influence nodulation.
Takeaway
The tep1 gene helps bacteria fight off a certain antibiotic and affects how well they can form nodules on alfalfa plants.
Methodology
The study involved creating a mutant strain of Sinorhizobium meliloti and testing its sensitivity to antibiotics and nodulation efficiency on alfalfa.
Limitations
The physiological functions of the closest SMc02161 homologs have not been experimentally tested.
Digital Object Identifier (DOI)
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