Highly Fluorescent Glutathione-coated Near Infrared Quantum Dots for Imaging
Author Information
Author(s): Jin Takashi, Fujii Fumihiko, Komai Yutaka, Seki Junji, Seiyama Akitoshi, Yoshioka Yoshichika
Primary Institution: WPI Immunology Frontier Research Center, Osaka University
Hypothesis
Can glutathione-coated near-infrared quantum dots be effectively used for in vivo fluorescence imaging?
Conclusion
The study successfully developed glutathione-coated near-infrared quantum dots that are highly fluorescent and stable for in vivo imaging.
Supporting Evidence
- The glutathione-coated quantum dots showed a quantum yield of 22% in PBS buffer.
- The hydrodynamic diameter of the quantum dots was found to be less than 10 nm.
- The cellular uptake of the quantum dots was observed in HeLa and HEK 293 cells.
- The viability of HeLa cells remained over 80% after treatment with the quantum dots.
- The viability of HEK 293 cells decreased to about 50% after treatment with the quantum dots.
Takeaway
Scientists made tiny glowing particles that can be used to see inside living things without hurting them. These particles are special because they stay bright and safe in water.
Methodology
The study involved synthesizing glutathione-coated near-infrared quantum dots and testing their fluorescence, stability, and cellular uptake.
Limitations
The cytotoxic effects varied between different cell types, and the long-term stability in physiological conditions needs further investigation.
Digital Object Identifier (DOI)
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