Cyclophilin A and Cholangiocarcinoma: A Study on Cell Growth
Author Information
Author(s): Obchoei Sumalee, Weakley Sarah M, Wongkham Sopit, Wongkham Chaisiri, Sawanyawisuth Kanlayanee, Yao Qizhi, Chen Changyi
Primary Institution: Baylor College of Medicine, Houston, Texas, USA; Khon Kaen University, Khon Kaen, Thailand
Hypothesis
The study investigates the role of Cyclophilin A (CypA) in promoting cell proliferation and tumor growth in liver fluke-associated cholangiocarcinoma (CCA).
Conclusion
CypA is upregulated in CCA tissues and enhances cell proliferation, making it a potential therapeutic target.
Supporting Evidence
- CypA was upregulated in 68% of CCA tumor tissues.
- Silencing CypA significantly suppressed cell proliferation in several CCA cell lines.
- Inhibition of CypA activity using cyclosporin A decreased cell proliferation.
- Overexpression of CypA resulted in 30% to 35% increases in proliferation of CCA cell lines.
- CypA silence led to a 43% reduction in tumor weight in mice.
Takeaway
Cyclophilin A helps cancer cells grow faster, and if we can stop it, we might slow down the cancer.
Methodology
The study used real-time RT-PCR, Western blot, immunohistochemistry, and in vivo experiments with nude mice to assess CypA's role in CCA.
Participant Demographics
Patients with cholangiocarcinoma from Northeast Thailand.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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