Human Tumor Xenografts in Mice
Author Information
Author(s): S. Aamdall, Q. Fodstad, J.M. Nesland, A. Pihll
Primary Institution: Norsk Hydro's Institute for Cancer Research, The Norwegian Radium Hospital
Hypothesis
Do human tumors of different histological types, grafted under the renal capsule of immunocompetent mice, contain viable, proliferating tumor cells and retain the characteristics of the grafted tumor tissue?
Conclusion
Human tumors transplanted under the renal capsule of immunocompetent mice retain the morphological and functional characteristics of the original xenografts.
Supporting Evidence
- All tumors studied, except one, increased in size during the 6-day period after transplantation.
- Mitoses were detected in all tumors, except one, showing that the tumor cells were proliferating.
- The grafted tumor tissue retained the ability to produce characteristic substances after growth under the renal capsule.
- Single cell suspensions from the grafts were able to form colonies in soft agar.
- Out of 12 different tumors studied, 10 grew subcutaneously in new hosts after re-implantation.
Takeaway
Scientists put human tumors in mice to see if they grow and stay the same. They found that the tumors did grow and kept their important features.
Methodology
Human tumor lines were grafted under the renal capsule of immunocompetent mice, and their growth and characteristics were evaluated over a 6-day period.
Potential Biases
Potential bias due to the use of previously established tumor lines rather than fresh patient samples.
Limitations
The study may not reflect the behavior of tumors directly from patients as it used serially transplanted tumors.
Participant Demographics
B6D2Fl mice, 6-12 weeks old, were used as host animals.
Statistical Information
P-Value
p<0.03
Statistical Significance
p<0.03
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