Transcriptional response of Mexican axolotls to Ambystoma tigrinum virus (ATV) infection
2008

Mexican Axolotls' Immune Response to ATV Infection

Sample size: 12 publication Evidence: moderate

Author Information

Author(s): Cotter Jennifer D, Storfer Andrew, Page Robert B, Beachy Christopher K, Voss S Randal

Primary Institution: Washington State University

Hypothesis

How do Mexican axolotls respond at the genetic level to Ambystoma tigrinum virus (ATV) infection?

Conclusion

Mexican axolotls mount a strong innate immune response to ATV, but lack the lymphocyte proliferation needed to clear the virus effectively.

Supporting Evidence

  • 158 genes were up-regulated and 105 down-regulated in response to ATV infection.
  • Significant gene expression changes were detected as early as 24 hours post-infection.
  • Genes related to immune response and inflammation were among the most affected.

Takeaway

When axolotls get sick from a virus, their bodies try really hard to fight it off, but they can't make enough of the special cells that help them get better.

Methodology

The study used a custom microarray gene chip to analyze gene expression in axolotls infected with ATV at various time points.

Limitations

The study did not observe lymphocyte proliferation in response to ATV, which may limit understanding of the immune response.

Participant Demographics

Inbred A. mexicanum eggs from a single full-sib mating were used.

Statistical Information

P-Value

p ≤ 0.01

Statistical Significance

p ≤ 0.01

Digital Object Identifier (DOI)

10.1186/1471-2164-9-493

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