Checkpoint Effects and Telomere Amplification During DNA Re-Replication in Fission Yeast
Author Information
Author(s): Katie L Mickle, Anna Oliva, Joel A Huberman, Janet Leatherwood
Primary Institution: Department of Microbiology and Molecular Genetics, SUNY at Stony Brook
Hypothesis
How do checkpoint pathways modulate re-replication in fission yeast?
Conclusion
Re-replication in fission yeast is largely independent of the replication and DNA-damage checkpoint pathways mediated by Cds1 and Rad3.
Supporting Evidence
- Over-expressing a non-phosphorylatable form of Cdc18 drove re-replication of DNA sequences genome-wide.
- Regions near telomeres were particularly susceptible to re-replication.
- The patterns of re-replication in cds1Δ and wild-type cells were nearly identical.
Takeaway
This study found that when certain proteins are over-expressed in fission yeast, it can lead to the re-replication of DNA across large regions of the genome, especially near telomeres.
Methodology
Microarrays were used to analyze DNA copy number changes in wild-type and checkpoint-mutant cells of fission yeast.
Limitations
The results for rad3Δ cells are preliminary due to their ability to divide during re-replication.
Digital Object Identifier (DOI)
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