Automated Library Preparation for High Throughput Sequencing
Author Information
Author(s): Erik Borgström, Sverker Lundin, Joakim Lundeberg, Michael N. Nitabach
Primary Institution: Science for Life Laboratory, Royal Institute of Technology (KTH), School of Biotechnology, Division of Gene Technology, Solna, Sweden
Hypothesis
Can an automated method for library preparation and size selection improve throughput and quality in DNA sequencing?
Conclusion
The automated method generates libraries with quality comparable to manual preparations while significantly increasing throughput.
Supporting Evidence
- The automated method can process 12 samples in about 4 hours.
- Quality scores and pass filter rates of the automated libraries are comparable to those prepared manually.
- The method allows for adjustable sample size distribution for various applications.
Takeaway
This study shows a new way to prepare DNA samples for sequencing that is faster and just as good as the old method.
Methodology
The study presents an automated protocol for library preparation using selective precipitation of DNA on paramagnetic beads.
Limitations
The automated size selection method produces a wider size distribution compared to manual gel separation.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website