Producing Proteins in Engineered Storage Organelles
Author Information
Author(s): Margarita Torrent, Blanca Llompart, Sabine Lasserre-Ramassamy, Immaculada Llop-Tous, Miriam Bastida, Pau Marzabal, Ann Westerholm-Parvinen, Markku Saloheimo, Peter B Heifetz, Dolors Ludevid
Primary Institution: Departament de Genètica Molecular, Consorci CSIC-IRTA
Hypothesis
Can the Zera domain from maize enhance recombinant protein production in non-plant eukaryotic cells?
Conclusion
The Zera sequence allows for efficient production of recombinant proteins by encapsulating them in ER-derived organelles without disrupting cell metabolism.
Supporting Evidence
- Zera fusions were shown to accumulate in PB-like organelles across various eukaryotic systems.
- The study demonstrated that Zera fusions do not affect the viability of the host cells.
- High levels of recombinant proteins were achieved in both plant and animal cells using Zera fusions.
Takeaway
Scientists found a way to help cells make more proteins by putting them in special storage bags inside the cells, which keeps them safe and helps them grow better.
Methodology
The study involved creating fusions of the Zera domain with various proteins and expressing them in different eukaryotic cell types to observe protein accumulation in induced organelles.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website